Hotspot

ccsA

SSC · 112326-113303 · 978 bp

Back to Origanum

Pi 0.000682

MarkerSeek score 35.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 1
pi: 0.000682
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGAATTTCATTTCCGATTTCGA	TCCTATTGGTAAGAATCCCCC	31.8	47.6	54.7	56.2	1061-1064 bp	1.0	40.1
ccsA_p2	TGAATTTCATTTCCGATTTCGA	TTCCTATTGGTAAGAATCCCCC	31.8	45.5	54.7	56.9	1062-1065 bp	1.0	40.1
ccsA_p3	ATGAATTTCATTTCCGATTTCGA	TCCTATTGGTAAGAATCCCCC	30.4	47.6	55.3	56.2	1062-1065 bp	1.0	40.1
ccsA_p4	TGAATTTCATTTCCGATTTCGAT	TCCTATTGGTAAGAATCCCCC	30.4	47.6	55.3	56.2	1061-1064 bp	1.0	40.1
ccsA_p5	TGAATTTCATTTCCGATTTCG	TCCTATTGGTAAGAATCCCCC	33.3	47.6	53.2	56.2	1061-1064 bp	1.0	40.1