Hotspot

rpl32-trnL-UAG

SSC · 111422-112143 · 722 bp

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Pi 0.000925

MarkerSeek score 52.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 722
variable_sites: 1
pi: 0.000925
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGGAAAGGTTATTGGGCGG	GATGATATGCCATGCCGCTA	50.0	50.0	58.2	58.0	917-923 bp	1.0	79.2
rpl32-trnL-UAG_p2	AAGGAAAGGTTATTGGGCGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	864-870 bp	1.0	78.4
rpl32-trnL-UAG_p3	AAGGAAAGGTTATTGGGCGG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.2	57.8	857-863 bp	1.0	77.9
rpl32-trnL-UAG_p4	AAGGAAAGGTTATTGGGCGG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.2	57.8	863-869 bp	1.0	77.9
rpl32-trnL-UAG_p5	AAGGAAAGGTTATTGGGCGG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.2	57.8	862-868 bp	1.0	77.9