Hotspot

pafII-cemA

LSC · 60018-60646 · 629 bp

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Pi 0

MarkerSeek score 59.60

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: LSC
length_bp: 629
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	TGCGCGTACCAATTGAAGTA	GAAATCCACCAGGGCAGAAA	45.0	50.0	57.9	58.1	721-726 bp	1.0	79.3
pafII-cemA_p2	CTTGCGCGTACCAATTGAAG	GAAATCCACCAGGGCAGAAA	50.0	50.0	58.1	58.1	723-728 bp	1.0	79.0
pafII-cemA_p3	GAACAAAAAGCCGCGGAATT	GAAATCCACCAGGGCAGAAA	45.0	50.0	58.2	58.1	752-757 bp	1.0	78.1
pafII-cemA_p4	TTTCTTGCGCGTACCAATTG	GAAATCCACCAGGGCAGAAA	45.0	50.0	57.7	58.1	726-731 bp	1.0	77.3
pafII-cemA_p5	TGCGCGTACCAATTGAAGTA	TCCACCAGGGCAGAAATACT	45.0	50.0	57.9	58.3	717-722 bp	1.0	77.3