Hotspot

rpoB-trnC-GCA

LSC · 26822-27953 · 1132 bp

Back to Origanum

Pi 0.00177

MarkerSeek score 46.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1132
variable_sites: 3
pi: 0.00177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGTTCTTCTGCCAAACCCTG	AACAAGCGGGTTTGATTCCT	50.0	45.0	58.0	58.0	1586-1597 bp	1.0	80.9
rpoB-trnC-GCA_p2	GTTCTTCTGCCAAACCCTGA	AACAAGCGGGTTTGATTCCT	50.0	45.0	58.0	58.0	1585-1596 bp	1.0	80.9
rpoB-trnC-GCA_p3	AGTTCTTCTGCCAAACCCTG	AGTAAGAGCCCATTCCCCTT	50.0	50.0	58.0	58.0	1831-1842 bp	1.0	80.7
rpoB-trnC-GCA_p4	GTTCTTCTGCCAAACCCTGA	AGTAAGAGCCCATTCCCCTT	50.0	50.0	58.0	58.0	1830-1841 bp	1.0	80.7
rpoB-trnC-GCA_p5	CCGCCTACACAAGCAAATTG	AACAAGCGGGTTTGATTCCT	50.0	45.0	58.0	58.0	2186-2197 bp	1.0	80.6