Hotspot

ycf1

SSC · 123275-128695 · 5421 bp

Back to Oreosalsola

Pi 0.0096

MarkerSeek score 58.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5421
variable_sites: 52
pi: 0.0096
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGATTGCTATCCCAGGCTTC	GGTTCCATGCGTGTTCAAAG	55.0	50.0	58.1	57.9	275 bp	1.0	80.9
ycf1_p2	GGATTGCTATCCCAGGCTTC	CAAAGGTTCCATGCGTGTTC	55.0	50.0	58.1	57.9	279 bp	1.0	80.8
ycf1_p3	GGATTGCTATCCCAGGCTTC	ACCGGGCGAAATCGAATTTA	55.0	45.0	58.1	58.0	339 bp	1.0	80.7
ycf1_p4	GGATTGCTATCCCAGGCTTC	ATCAAAGGTTCCATGCGTGT	55.0	45.0	58.1	58.1	281 bp	1.0	80.5
ycf1_p5	GGATTGCTATCCCAGGCTTC	ACGTTATCCACAAGAACCCG	55.0	50.0	58.1	57.9	318 bp	1.0	80.3