Hotspot

atpA

LSC · 9905-11428 · 1524 bp

Back to Oreosalsola

Pi 0.00525

MarkerSeek score 48.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 8
pi: 0.00525
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	2438-2440 bp	1.0	80.4
atpA_p2	AGCCTTCCAAGCTAACGATG	TCAAGTTTGGTTCGGGAAGG	50.0	50.0	58.0	58.0	2403-2405 bp	1.0	80.3
atpA_p3	CCTAGCCTTCCAAGCTAACG	GTAATTGAGCGTGAGAGCCA	55.0	50.0	58.1	58.0	2441-2443 bp	1.0	80.2
atpA_p4	AGCCTTCCAAGCTAACGATG	GAAGAACTTCGCGGAAAAGC	50.0	50.0	58.0	58.0	2282-2284 bp	1.0	80.2
atpA_p5	CCTAGCCTTCCAAGCTAACG	TCAAGTTTGGTTCGGGAAGG	55.0	50.0	58.1	58.0	2406-2408 bp	1.0	80.1