Hotspot

psaC-ndhE

SSC · 116921-117264 · 344 bp

Back to Oreosalsola

Pi 0.0203

MarkerSeek score 65.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 344
variable_sites: 7
pi: 0.0203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CAAGCTCGGACACATTGAGT	GCTATTGGACCGGCTATTGT	50.0	50.0	58.2	57.7	490-502 bp	1.0	77.5
psaC-ndhE_p2	AATACATCTGTGGGGCAAGC	GCTATTGGACCGGCTATTGT	50.0	50.0	58.2	57.7	505-517 bp	1.0	77.2
psaC-ndhE_p3	GCTCGGACACATTGAGTACA	GCTATTGGACCGGCTATTGT	50.0	50.0	57.6	57.7	487-499 bp	1.0	76.2
psaC-ndhE_p4	CAAGCTCGGACACATTGAGT	AGCTATTGGACCGGCTATTG	50.0	50.0	58.2	57.4	491-503 bp	1.0	75.1
psaC-ndhE_p5	CAAGCTCGGACACATTGAGT	CAGCTATTGGACCGGCTATT	50.0	50.0	58.2	57.4	492-504 bp	1.0	75.1