Hotspot

ndhF-rpl32

SSC · 111441-112547 · 1107 bp

Back to Oreosalsola

Pi 0.0127

MarkerSeek score 56.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1107
variable_sites: 14
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CAAAGGAACGGGAAGTGGAA	CGAATGCGCTTTTTGTAAATCG	50.0	40.9	58.0	57.9	1214-1216 bp	1.0	64.0
ndhF-rpl32_p2	CAAAGGAACGGGAAGTGGAA	TGCGCTTTTTGTAAATCGAAGT	50.0	36.4	58.0	57.8	1210-1212 bp	1.0	63.3
ndhF-rpl32_p3	GAACGGGAAGTGGAAGGAAG	CGAATGCGCTTTTTGTAAATCG	55.0	40.9	58.2	57.9	1209-1211 bp	1.0	62.6
ndhF-rpl32_p4	GAACGGGAAGTGGAAGGAAG	TGCGCTTTTTGTAAATCGAAGT	55.0	36.4	58.2	57.8	1205-1207 bp	1.0	62.0
ndhF-rpl32_p5	AAAGGAACGGGAAGTGGAAG	CGAATGCGCTTTTTGTAAATCG	50.0	40.9	57.7	57.9	1213-1215 bp	1.0	62.0