Hotspot

trnG-UCC-trnfM-CAU

LSC · 36420-36625 · 206 bp

Back to Oreosalsola

Pi 0.0343

MarkerSeek score 73.10

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 206
variable_sites: 7
pi: 0.0343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	GTTCAAATCCTGTCTCCGCA	50.0	50.0	57.8	58.2	271-278 bp	1.0	79.7
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	305-312 bp	1.0	79.2
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	304-311 bp	1.0	79.2
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TTATCCATTCGTTCCCAGCC	50.0	50.0	57.8	57.7	359-366 bp	1.0	77.9
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TCAAATCCTGTCTCCGCAAA	50.0	45.0	57.8	57.4	269-276 bp	1.0	76.8