Hotspot

rpoB-trnC-GCA

LSC · 26453-27568 · 1116 bp

Back to Oreosalsola

Pi 0.00643

MarkerSeek score 64.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 7
pi: 0.00643
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCCATCCCGTAGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.1	58.1	1173-1200 bp	1.0	80.8
rpoB-trnC-GCA_p2	CCTCATTTCCATCCCGTAGC	AAAGGATTTGCAGTCCTCCG	55.0	50.0	58.1	58.2	1154-1181 bp	1.0	80.1
rpoB-trnC-GCA_p3	CATCCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.7	58.1	1178-1205 bp	1.0	79.3
rpoB-trnC-GCA_p4	CATCCCCTCATTTCCATCCC	AAAGGATTTGCAGTCCTCCG	55.0	50.0	57.7	58.2	1159-1186 bp	1.0	78.6
rpoB-trnC-GCA_p5	CCCTCATTTCCATCCCGTAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.4	58.1	1174-1201 bp	1.0	77.3