Hotspot

trnG-GCC-trnR-UCU

LSC · 9619-9743 · 125 bp

Back to Oreosalsola

Pi 0.08
MarkerSeek score 80.70
Primer available yes
Label trnG-GCC-trnR-UCU

Feature Metadata

label_name
trnG-GCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
125
variable_sites
10
pi
0.08
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG TGGAAATAAATTGCGTCCAATAGG 50.0 37.5 58.0 57.9 262 bp 1.0 49.7
trnG-GCC-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG TGGAAATAAATTGCGTCCAATAGG 55.0 37.5 58.1 57.9 265 bp 1.0 49.4
trnG-GCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG TGGAAATAAATTGCGTCCAA 50.0 35.0 58.0 53.8 262 bp 1.0 48.9
trnG-GCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG TGGAAATAAATTGCGTCCAA 55.0 35.0 58.1 53.8 265 bp 1.0 48.6
trnG-GCC-trnR-UCU_p5 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTATGTCCT 50.0 40.9 58.0 55.6 221 bp 1.0 48.4