Hotspot

trnS-GCU-trnG-GCC

LSC · 7967-8823 · 857 bp

Back to Oreosalsola

Pi 0.0129

MarkerSeek score 58.20

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 857
variable_sites: 11
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ACGGAAAGAGAGGGATTCGA	CGGAATAGTAATCAAACCGGGA	50.0	45.5	58.2	57.9	1031-1036 bp	1.0	63.2
trnS-GCU-trnG-GCC_p2	ACGGAAAGAGAGGGATTCGA	TCGGAATAGTAATCAAACCGGG	50.0	45.5	58.2	57.9	1032-1037 bp	1.0	63.2
trnS-GCU-trnG-GCC_p3	CGAATGACTCGTACAACGGA	CGGAATAGTAATCAAACCGGGA	50.0	45.5	57.7	57.9	1001-1006 bp	1.0	62.4
trnS-GCU-trnG-GCC_p4	CGAATGACTCGTACAACGGA	TCGGAATAGTAATCAAACCGGG	50.0	45.5	57.7	57.9	1002-1007 bp	1.0	62.4
trnS-GCU-trnG-GCC_p5	ACGGAAAGAGAGGGATTCGA	CGGAATAGTAATCAAACCGGG	50.0	47.6	58.2	56.5	1031-1036 bp	1.0	59.7