Hotspot

rbcL

LSC · 56058-57521 · 1464 bp

Back to Oreorchis

Pi 0.00273

MarkerSeek score 50.50

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1464
variable_sites: 6
pi: 0.00273
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCAAAGGTTTCATTCACGCC	ATTGGGCCAAGTTTCATTGC	45.0	45.0	56.9	57.5	1592 bp	1.0	67.9
rbcL_p2	TCAAAGGTTTCATTCACGCC	GGGCCAAGTTTCATTGCAAT	45.0	45.0	56.9	57.5	1589 bp	1.0	67.9
rbcL_p3	CAAAGGTTTCATTCACGCCT	ATTGGGCCAAGTTTCATTGC	45.0	45.0	56.9	57.5	1591 bp	1.0	67.8
rbcL_p4	CAAAGGTTTCATTCACGCCT	GGGCCAAGTTTCATTGCAAT	45.0	45.0	56.9	57.5	1588 bp	1.0	67.8
rbcL_p5	GTCAAAGGTTTCATTCACGCC	GGGCCAAGTTTCATTGCAAT	47.6	45.0	58.3	57.5	1590 bp	1.0	65.8