Hotspot

trnN-GUU-trnR-ACG

IRa · 132999-133582 · 584 bp

Back to Oreorchis

Pi 0.0126

MarkerSeek score 66.40

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 584
variable_sites: 11
pi: 0.0126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	725 bp	1.0	79.5
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	TTTGAAGGTACTCCCGGGAA	55.0	50.0	58.0	58.3	779 bp	1.0	79.0
trnN-GUU-trnR-ACG_p3	CCGTTGTTCCTCAGTAGCTC	TTTTGAAGGTACTCCCGGGA	55.0	50.0	58.0	58.3	780 bp	1.0	79.0
trnN-GUU-trnR-ACG_p4	CGGTTGGCTGTTAACTGACT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	695 bp	1.0	78.6
trnN-GUU-trnR-ACG_p5	GCTGTTAACTGACTGGTCGT	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	689 bp	1.0	78.3