Hotspot

ccsA-ndhD

SSC · 118458-118676 · 219 bp

Back to Oreorchis

Pi 0.0457

MarkerSeek score 79.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 219
variable_sites: 15
pi: 0.0457
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACGAATTCGGCATTTGTAGC	TCCTGATTTTGTTCTCCCGT	45.0	45.0	57.2	56.8	412-475 bp	1.0	66.1
ccsA-ndhD_p2	ACGAATTCGGCATTTGTAGC	TCGGTTGACAAGGTACAAGT	45.0	45.0	57.2	56.7	390-453 bp	1.0	65.9
ccsA-ndhD_p3	ACGAATTCGGCATTTGTAGC	TCCTGATTTTGTTCTCCCGTT	45.0	42.9	57.2	57.5	412-475 bp	1.0	64.0
ccsA-ndhD_p4	CGAATTCGGCATTTGTAGCT	TCCTGATTTTGTTCTCCCGT	45.0	45.0	56.9	56.8	411-474 bp	1.0	63.8
ccsA-ndhD_p5	ACGAATTCGGCATTTGTAGC	TCGGTTGACAAGGTACAAGTT	45.0	42.9	57.2	57.5	390-453 bp	1.0	63.8