Hotspot

trnR-ACG-trnN-GUU

IRb · 111367-111950 · 584 bp

Back to Oreorchis

Pi 0.0126

MarkerSeek score 66.40

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 584
variable_sites: 11
pi: 0.0126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	725 bp	1.0	79.5
trnR-ACG-trnN-GUU_p2	TTTGAAGGTACTCCCGGGAA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.3	58.0	779 bp	1.0	79.0
trnR-ACG-trnN-GUU_p3	TTTTGAAGGTACTCCCGGGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.3	58.0	780 bp	1.0	79.0
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	CGGTTGGCTGTTAACTGACT	55.0	50.0	57.8	58.1	695 bp	1.0	78.6
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	689 bp	1.0	78.3