Hotspot

atpB-rbcL

LSC · 55220-56057 · 838 bp

Back to Oreorchis

Pi 0.0124

MarkerSeek score 69.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 15
pi: 0.0124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATCTTGCCCGGTGGAAAAAT	GGTGACATAAGTCCCTCCCT	45.0	55.0	58.1	58.1	985-1015 bp	1.0	81.4
atpB-rbcL_p2	TTTGAGCAATACGCCCTAGG	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.7	58.1	942-972 bp	1.0	79.5
atpB-rbcL_p3	GAGCAATACGCCCTAGGTTT	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.7	58.1	939-969 bp	1.0	79.5
atpB-rbcL_p4	ATCTTGCCCGGTGGAAAAAT	TGGTGACATAAGTCCCTCCC	45.0	55.0	58.1	58.4	986-1016 bp	1.0	79.0
atpB-rbcL_p5	TTTGAGCAATACGCCCTAGG	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.7	58.4	943-973 bp	1.0	77.1