Hotspot

psbM-trnD-GUC

LSC · 29900-31025 · 1126 bp

Back to Oreorchis

Pi 0.00836

MarkerSeek score 64.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1126
variable_sites: 14
pi: 0.00836
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1278-1288 bp	1.0	78.8
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1226-1236 bp	1.0	78.8
psbM-trnD-GUC_p3	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.6	57.9	1225-1235 bp	1.0	77.8
psbM-trnD-GUC_p4	AAATGAACAGCGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.5	57.9	1228-1238 bp	1.0	77.1
psbM-trnD-GUC_p5	AATGAACAGCGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.5	57.9	1227-1237 bp	1.0	77.1