Hotspot

rpoB-trnC-GCA

LSC · 26981-28265 · 1285 bp

Back to Oreorchis

Pi 0.0119

MarkerSeek score 52.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 23
pi: 0.0119
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCAAATTAAGACGGCGGGTA	AGTATCGACAGACCTAGCCC	50.0	55.0	58.0	58.0	1718-1719 bp	1.0	81.0
rpoB-trnC-GCA_p2	CCAAATTAAGACGGCGGGTA	GTATCGACAGACCTAGCCCT	50.0	55.0	58.0	58.0	1717-1718 bp	1.0	81.0
rpoB-trnC-GCA_p3	CCCCTACACAGGCAAATTGT	AGTATCGACAGACCTAGCCC	50.0	55.0	58.1	58.0	2160-2161 bp	1.0	80.4
rpoB-trnC-GCA_p4	CCCCTACACAGGCAAATTGT	GTATCGACAGACCTAGCCCT	50.0	55.0	58.1	58.0	2159-2160 bp	1.0	80.4
rpoB-trnC-GCA_p5	CCAAATTAAGACGGCGGGTA	GCCCTACGGGTTCTCAAAAT	50.0	50.0	58.0	57.9	1740-1741 bp	1.0	80.1