Hotspot

trnS-GCU-trnG-GCC

LSC · 8351-9235 · 885 bp

Back to Oreorchis

Pi 0.0144

MarkerSeek score 66.00

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 18
pi: 0.0144
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	883-954 bp	1.0	75.6
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCACT	50.0	42.9	58.0	58.2	882-953 bp	1.0	75.6
trnS-GCU-trnG-GCC_p3	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCAC	50.0	45.0	58.0	56.8	883-954 bp	1.0	75.6
trnS-GCU-trnG-GCC_p4	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCAC	50.0	45.0	58.0	56.8	882-953 bp	1.0	75.6
trnS-GCU-trnG-GCC_p5	CAATCCGACGCTTTAGTCCA	AACGAATCGCACTTTTACCAC	50.0	42.9	58.0	57.5	884-955 bp	1.0	73.3