Hotspot

ycf1

SSC · 126651-132401 · 5751 bp

Back to Oreocnide

Pi 0.0021
MarkerSeek score 44.60
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5751
variable_sites
59
pi
0.0021
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGGATCAGCTTGAACTCTTTTCA AAAATTCGGGATCTCTTTCT 39.1 35.0 58.0 52.1 273 bp 1.0 43.5
ycf1_p2 AGGATCAGCTTGAACTCTTTTCA AAATTCGGGATCTCTTTCTT 39.1 35.0 58.0 52.1 272 bp 1.0 43.5
ycf1_p3 AGGATCAGCTTGAACTCTTTTCA AAAATTCGGGATCTCTTTCTT 39.1 33.3 58.0 53.1 273 bp 1.0 43.5
ycf1_p4 TGTTAGGATCAGCTTGAACTCT AAAATTCGGGATCTCTTTCT 40.9 35.0 56.8 52.1 277 bp 1.0 43.5
ycf1_p5 TGTTAGGATCAGCTTGAACTCT AAATTCGGGATCTCTTTCTT 40.9 35.0 56.8 52.1 276 bp 1.0 43.5