Hotspot

atpA

LSC · 10441-11964 · 1524 bp

Back to Oreocnide

Pi 0.000646

MarkerSeek score 30.30

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 4
pi: 0.000646
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	2429-2435 bp	1.0	80.3
atpA_p2	ACCCTTAGCCTTCCAAGCTA	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	2435-2441 bp	1.0	80.3
atpA_p3	AGCCTTCCAAGCTAACGATG	ATTCGTGTTTGGTTCGGGAA	50.0	45.0	58.0	58.0	2396-2402 bp	1.0	80.2
atpA_p4	ACCCTTAGCCTTCCAAGCTA	ATTCGTGTTTGGTTCGGGAA	50.0	45.0	58.0	58.0	2402-2408 bp	1.0	80.1
atpA_p5	AGCCTTCCAAGCTAACGATG	AAAAAGCCCAATCCCGCTTA	50.0	45.0	58.0	58.1	2239-2245 bp	1.0	79.9