Hotspot

rpl32-trnL-UAG

SSC · 115970-116829 · 860 bp

Back to Oreocnide

Pi 0.0102
MarkerSeek score 54.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
860
variable_sites
40
pi
0.0102
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCATTGAAAGCCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 964-1016 bp 1.0 83.1
rpl32-trnL-UAG_p2 GGCAGCATTGAAAGCCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 965-1017 bp 1.0 83.1
rpl32-trnL-UAG_p3 GGCAGCATTGAAAGCCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 974-1026 bp 1.0 82.1
rpl32-trnL-UAG_p4 GGCAGCATTGAAAGCCTTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.8 57.8 973-1025 bp 1.0 81.6
rpl32-trnL-UAG_p5 GGCAGCATTGAAAGCCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.8 57.8 972-1024 bp 1.0 81.6