Hotspot

psbE-petL

LSC · 66620-67848 · 1229 bp

Back to Oreocnide

Pi 0.00329

MarkerSeek score 57.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 22
pi: 0.00329
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.9	56.3	1364-1377 bp	1.0	69.0
psbE-petL_p2	ACGAATAACCAACCCGCAAT	AGCTGCTAGTAGAAAACCGA	45.0	45.0	58.2	56.3	1373-1386 bp	1.0	68.1
psbE-petL_p3	TGCTGACGAATAACCAACCC	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.2	56.3	1378-1391 bp	1.0	67.9
psbE-petL_p4	CGAATAACCAACCCGCAATG	AGCTGCTAGTAGAAAACCGA	50.0	45.0	57.8	56.3	1372-1385 bp	1.0	67.9
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.2	56.3	1366-1379 bp	1.0	67.5