Hotspot

petA-psbJ

LSC · 64858-65839 · 982 bp

Back to Oreocnide

Pi 0.00438

MarkerSeek score 54.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 982
variable_sites: 22
pi: 0.00438
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATTACGCGTACAAGGCCTTT	TCTTGTGATCGGTTTAATCGGT	45.0	40.9	57.9	57.8	1164-1206 bp	1.0	63.1
petA-psbJ_p2	TTACGCGTACAAGGCCTTTT	TCTTGTGATCGGTTTAATCGGT	45.0	40.9	58.1	57.8	1163-1205 bp	1.0	63.0
petA-psbJ_p3	ATTACGCGTACAAGGCCTTT	ACTGGTATTCTTGTGATCGGT	45.0	42.9	57.9	56.7	1172-1214 bp	1.0	62.6
petA-psbJ_p4	TTACGCGTACAAGGCCTTTT	ACTGGTATTCTTGTGATCGGT	45.0	42.9	58.1	56.7	1171-1213 bp	1.0	62.5
petA-psbJ_p5	ACAAGGCCTTTTGCTCTTCT	TCTTGTGATCGGTTTAATCGGT	45.0	40.9	57.6	57.8	1155-1197 bp	1.0	61.0