Hotspot

rpoB-trnC-GCA

LSC · 27079-28291 · 1213 bp

Back to Oreocnide

Pi 0.00511

MarkerSeek score 50.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1213
variable_sites: 21
pi: 0.00511
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ACATTTCCTCATTTCCGCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.5	58.1	1295-1322 bp	1.0	77.3
rpoB-trnC-GCA_p2	ACATTTCCTCATTTCCGCCC	TTGTTAATCAGGCGACACCC	50.0	50.0	58.5	58.2	1313-1340 bp	1.0	76.5
rpoB-trnC-GCA_p3	AAGCCCCAATCAATGAACCT	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.4	58.1	1359-1386 bp	1.0	76.4
rpoB-trnC-GCA_p4	AAGCCCCAATCAATGAACCT	TTGTTAATCAGGCGACACCC	45.0	50.0	57.4	58.2	1377-1404 bp	1.0	75.6
rpoB-trnC-GCA_p5	ACATTTCCTCATTTCCGCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.5	58.4	1277-1304 bp	1.0	75.1