Hotspot

atpF-atpH

LSC · 13337-13794 · 458 bp

Back to Oreocnide

Pi 0.00399

MarkerSeek score 53.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 458
variable_sites: 7
pi: 0.00399
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GATGGCCAATAACCCAAGGA	TGGTTGTGGCATTAGCACTT	50.0	45.0	57.5	58.0	545-558 bp	1.0	81.0
atpF-atpH_p2	GGATGGCCAATAACCCAAGG	TGGTTGTGGCATTAGCACTT	55.0	45.0	58.6	58.0	546-559 bp	1.0	79.9
atpF-atpH_p3	GATGGCCAATAACCCAAGGA	TGGACTGGTTGTGGCATTAG	50.0	50.0	57.5	57.8	550-563 bp	1.0	79.4
atpF-atpH_p4	TGGCCAATAACCCAAGGAAA	TGGTTGTGGCATTAGCACTT	45.0	45.0	57.3	58.0	543-556 bp	1.0	79.0
atpF-atpH_p5	TTAAACCCGAAACTTCCGGC	TGGTTGTGGCATTAGCACTT	50.0	45.0	58.8	58.0	566-579 bp	1.0	78.3