Hotspot

atpA-atpF

LSC · 11965-12032 · 68 bp

Back to Oreocnide

Pi 0.0372

MarkerSeek score 63.00

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 68
variable_sites: 5
pi: 0.0372
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	AATTTCATCGGCTCGAAGGG	ACAAGGAGCTCTAGGAACTCT	50.0	47.6	58.3	57.5	203-206 bp	1.0	68.8
atpA-atpF_p2	AATTTCATCGGCTCGAAGGG	AGGAGCTCTAGGAACTCTGA	50.0	50.0	58.3	56.5	200-203 bp	1.0	68.4
atpA-atpF_p3	ACTTGAAGTACGGTACCGGT	ACAAGGAGCTCTAGGAACTCT	50.0	47.6	58.4	57.5	280-283 bp	1.0	67.5
atpA-atpF_p4	ACTTGAAGTACGGTACCGGT	AGGAGCTCTAGGAACTCTGA	50.0	50.0	58.4	56.5	277-280 bp	1.0	67.2
atpA-atpF_p5	TCGCCTACTTGAAGTACGGT	ACAAGGAGCTCTAGGAACTCT	50.0	47.6	58.5	57.5	286-289 bp	1.0	66.8