Hotspot

rps16-trnQ-UUG

LSC · 6346-7168 · 823 bp

Back to Oreocnide

Pi 0.00681

MarkerSeek score 60.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 823
variable_sites: 28
pi: 0.00681
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	818-896 bp	1.0	84.9
rps16-trnQ-UUG_p2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	833-911 bp	1.0	83.0
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	832-910 bp	1.0	83.0
rps16-trnQ-UUG_p4	TGGGTGTAAGAATCCACAGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	852-930 bp	1.0	82.0
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.1	58.5	839-917 bp	1.0	81.2