Hotspot

ndhD

SSC · 115264-116766 · 1503 bp

Back to Oreocharis

Pi 0.00898

MarkerSeek score 44.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 41
pi: 0.00898
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CAATTCAGCAATTGTGGCGT	GATACCCTGGGACGGATGTA	45.0	55.0	57.9	58.0	2093-2165 bp	1.0	84.9
ndhD_p2	TTGGAGTTGGGACCCAAAAG	GATACCCTGGGACGGATGTA	50.0	55.0	57.9	58.0	2189-2261 bp	1.0	84.7
ndhD_p3	CAATTCAGCAATTGTGGCGT	GACCGAAGACTGTGTTGGTT	45.0	50.0	57.9	58.1	2045-2117 bp	1.0	84.7
ndhD_p4	TTGGAGTTGGGACCCAAAAG	GACCGAAGACTGTGTTGGTT	50.0	50.0	57.9	58.1	2141-2213 bp	1.0	84.4
ndhD_p5	CAATTCAGCAATTGTGGCGT	TGTGAATCTGCCTGTCCAAC	45.0	50.0	57.9	58.1	2017-2089 bp	1.0	84.3