Hotspot

rpl32-trnL-UAG

SSC · 113019-113887 · 869 bp

Back to Oreocharis

Pi 0.0157

MarkerSeek score 55.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 869
variable_sites: 39
pi: 0.0157
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GAAGGGGTATTGTGCATCGT	TTTAGGAGATGACATGCCGC	50.0	50.0	58.0	58.0	1010-1092 bp	1.0	86.7
rpl32-trnL-UAG_p2	GAAGGGGTATTGTGCATCGT	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	1426-1508 bp	1.0	86.0
rpl32-trnL-UAG_p3	GAAGGGGTATTGTGCATCGT	CGCTTGGGCCAATTATTGTG	50.0	50.0	58.0	58.1	1398-1480 bp	0.875	80.6
rpl32-trnL-UAG_p4	GAAGGGGTATTGTGCATCGT	AATAGCGCTTGGGCCAATTA	50.0	45.0	58.0	57.9	1472-1485 bp	0.75	71.4
rpl32-trnL-UAG_p5	GAAGGGGTATTGTGCATCGT	TAGCGCTTGGGCCAATTATT	50.0	45.0	58.0	57.9	1470-1483 bp	0.75	71.4