Hotspot

trnT-GGU-psbD

LSC · 32177-33491 · 1315 bp

Back to Oreocharis

Pi 0.0115

MarkerSeek score 57.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1315
variable_sites: 47
pi: 0.0115
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.3	53.0	1219-1384 bp	1.0	46.8
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTATAGTCA	55.0	40.0	58.1	53.0	1242-1407 bp	1.0	46.7
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.1	53.0	1245-1410 bp	1.0	46.7
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	TTACCAAGGGCTATAGTCAT	55.0	40.0	58.1	52.8	1241-1406 bp	1.0	46.7
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	TTACCAAGGGCTATAGTCAT	50.0	40.0	58.1	52.8	1244-1409 bp	1.0	46.7