Hotspot

atpA-atpF

LSC · 12023-12087 · 65 bp

Back to Oreocharis

Pi 0.0385

MarkerSeek score 64.90

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 10
pi: 0.0385
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CACTTGAAGTACGGTACCGG	GTAATTGAGCGTGAGAGCCA	55.0	50.0	58.0	58.0	676-682 bp	1.0	83.0
atpA-atpF_p2	CCACTTGAAGTACGGTACCG	GTAATTGAGCGTGAGAGCCA	55.0	50.0	58.0	58.0	677-683 bp	1.0	83.0
atpA-atpF_p3	CAATACCATCGCCCACTTGA	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	689-695 bp	1.0	82.7
atpA-atpF_p4	CACCCGCCATTACTTCATCA	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	725-731 bp	1.0	82.6
atpA-atpF_p5	CCAGGGCGTTTATAACACGA	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	908-914 bp	1.0	82.3