Hotspot

trnH-GUG-psbA

LSC · 82-410 · 329 bp

Back to Oreocharis

Pi 0.024

MarkerSeek score 55.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 329
variable_sites: 24
pi: 0.024
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	767-791 bp	1.0	83.0
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	781-805 bp	1.0	82.9
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	55.0	50.0	58.1	58.0	766-790 bp	1.0	82.9
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	780-804 bp	1.0	82.9
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	782-806 bp	1.0	82.8