primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.171606	82	410	767-791	1.000000	83.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.175967	82	410	781-805	1.000000	82.9
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.177364	82	410	766-790	1.000000	82.9
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	82	410	780-804	1.000000	82.9
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	82	410	782-806	1.000000	82.8
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8661	9425	834-986	1.000000	57.3
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8661	9425	833-985	1.000000	57.3
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8661	9425	837-989	1.000000	56.2
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8661	9425	836-988	1.000000	56.2
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8661	9425	835-987	1.000000	56.2
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10197	10349	146-263	1.000000	75.3
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10197	10349	149-266	1.000000	75.1
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10197	10349	152-269	1.000000	74.7
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10197	10349	155-272	1.000000	74.5
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	10197	10349	150-267	1.000000	72.9
atpA_p1	atpA	1	AGCCTTCCAAGCTAACGATG	TGGAAATGGAAGCCGATGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.072524	10499	12022	2143-2260	1.000000	85.5
atpA_p2	atpA	2	AGCCTTCCAAGCTAACGATG	GGAAATGGAAGCCGATGAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.073961	10499	12022	2142-2259	1.000000	85.5
atpA_p3	atpA	3	AGCCTTCCAAGCTAACGATG	GGAAGCCGATGAGTTTAGGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.074911	10499	12022	2136-2253	1.000000	85.5
atpA_p4	atpA	4	AGCCTTCCAAGCTAACGATG	GTAATTGAGCGTGAGAGCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039983	10499	12022	2360-2477	1.000000	85.4
atpA_p5	atpA	5	CCTAGCCTTCCAAGCTAACG	GTAATTGAGCGTGAGAGCCA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.068820	10499	12022	2363-2480	1.000000	85.1
atpA-atpF_p1	atpA-atpF	1	CACTTGAAGTACGGTACCGG	GTAATTGAGCGTGAGAGCCA	20	20	55.0	50.0	58.0	58.0	35.9	0.0	0.023447	12023	12087	676-682	1.000000	83.0
atpA-atpF_p2	atpA-atpF	2	CCACTTGAAGTACGGTACCG	GTAATTGAGCGTGAGAGCCA	20	20	55.0	50.0	58.0	58.0	32.2	0.0	0.023447	12023	12087	677-683	1.000000	83.0
atpA-atpF_p3	atpA-atpF	3	CAATACCATCGCCCACTTGA	GTAATTGAGCGTGAGAGCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047103	12023	12087	689-695	1.000000	82.7
atpA-atpF_p4	atpA-atpF	4	CACCCGCCATTACTTCATCA	GTAATTGAGCGTGAGAGCCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.047103	12023	12087	725-731	1.000000	82.6
atpA-atpF_p5	atpA-atpF	5	CCAGGGCGTTTATAACACGA	GTAATTGAGCGTGAGAGCCA	20	20	50.0	50.0	58.0	58.0	18.2	0.0	0.023082	12023	12087	908-914	1.000000	82.3
atpF-atpH_p1	atpF-atpH	1	CCAGTGGCCTAAAGAAACGA	AGGCAGAGGGAAAGATACGA	20	20	50.0	50.0	57.8	57.8	3.3	0.0	0.339633	13343	13726	526-544	1.000000	81.0
atpF-atpH_p2	atpF-atpH	2	TTAAATCCGAAACTCCCGGC	AGGCAGAGGGAAAGATACGA	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.419193	13343	13726	552-570	1.000000	80.2
atpF-atpH_p3	atpF-atpH	3	CCAGTGGCCTAAAGAAACGA	GAGGCAGAGGGAAAGATACG	20	20	50.0	55.0	57.8	57.5	3.3	0.0	0.696562	13343	13726	527-545	1.000000	78.1
atpF-atpH_p4	atpF-atpH	4	CCAGTGGCCTAAAGAAACGA	GCAGAGGGAAAGATACGAGG	20	20	50.0	55.0	57.8	57.5	3.3	0.0	0.696562	13343	13726	524-542	1.000000	78.1
atpF-atpH_p5	atpF-atpH	5	CCAGTGGCCTAAAGAAACGA	GGCAGAGGGAAAGATACGAG	20	20	50.0	55.0	57.8	57.5	3.3	0.0	0.696562	13343	13726	525-543	1.000000	78.1
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	58.3	53.0	0.0	6.7	5.273069	32177	33491	1219-1384	1.000000	46.8
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTATAGTCA	20	20	55.0	40.0	58.1	53.0	7.6	6.7	5.054330	32177	33491	1242-1407	1.000000	46.7
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTATAGTCA	20	20	50.0	40.0	58.1	53.0	0.0	6.7	5.076485	32177	33491	1245-1410	1.000000	46.7
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	TTACCAAGGGCTATAGTCAT	20	20	55.0	40.0	58.1	52.8	7.6	6.7	5.305690	32177	33491	1241-1406	1.000000	46.7
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	TTACCAAGGGCTATAGTCAT	20	20	50.0	40.0	58.1	52.8	0.0	6.7	5.327845	32177	33491	1244-1409	1.000000	46.7
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCATTGA	20	22	50.0	31.8	58.1	53.8	0.0	3.0	6.238188	49076	49739	716-731	1.000000	46.0
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	TGAAATACAAGATGCTCATTGA	20	22	55.0	31.8	58.0	53.8	10.3	3.0	6.188798	49076	49739	752-767	1.000000	45.7
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CTACATTGGGAATGGTCGGG	TGAAATACAAGATGCTCATTGA	20	22	55.0	31.8	58.0	53.8	0.0	3.0	6.218974	49076	49739	769-784	1.000000	45.6
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	TGCTACATTGGGAATGGTCG	TGAAATACAAGATGCTCATTGA	20	22	50.0	31.8	58.0	53.8	0.0	3.0	6.223250	49076	49739	771-786	1.000000	45.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GGATGGGATGCTACATTGGG	TGAAATACAAGATGCTCATTGA	20	22	55.0	31.8	58.1	53.8	0.0	3.0	6.271152	49076	49739	779-794	1.000000	45.5
psbT-pbf1_p1	psbT-pbf1	1	CGAGAACCACCTAAAGTTCCA	TCGAGGAACATGGGGACTAA	21	20	47.6	50.0	57.6	57.8	0.0	0.0	1.637541	75318	75382	107-118	1.000000	77.2
psbT-pbf1_p2	psbT-pbf1	2	CGAGAACCACCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	21	20	47.6	50.0	57.6	57.8	0.0	0.0	1.637541	75318	75382	108-119	1.000000	77.2
psbT-pbf1_p3	psbT-pbf1	3	CGAGAACCACCTAAAGTTCCA	CATTCGAGGAACATGGGGAC	21	20	47.6	55.0	57.6	58.3	0.0	0.0	1.727841	75318	75382	110-121	1.000000	76.3
psbT-pbf1_p4	psbT-pbf1	4	CGAGAACCACCTAAAGTTCCA	ATTCGAGGAACATGGGGACT	21	20	47.6	50.0	57.6	58.4	0.0	0.0	1.811536	75318	75382	109-120	1.000000	75.7
psbT-pbf1_p5	psbT-pbf1	5	TCGAGAACCACCTAAAGTTCC	TCGAGGAACATGGGGACTAA	21	20	47.6	50.0	57.3	57.8	0.0	0.0	1.914047	75318	75382	108-119	1.000000	75.0
rps19-rpl2_p1	rps19-rpl2	1	TGCTGCTGCAGAATAAACCA	AAAAGTTTGGGTAGAGCCGG	20	20	45.0	50.0	58.1	58.1	32.3	0.0	0.186019	84933	84987	802-804	1.000000	80.2
rps19-rpl2_p2	rps19-rpl2	2	TGCTGCTGCAGAATAAACCA	ATTACCTTCTGGGGAGGTCC	20	20	45.0	55.0	58.1	58.1	32.3	0.1	0.196957	84933	84987	884-886	1.000000	80.0
rps19-rpl2_p3	rps19-rpl2	3	TGCTGCTGCAGAATAAACCA	TGGGGAGGTCCGTTTGATAT	20	20	45.0	50.0	58.1	58.1	32.3	0.0	0.203859	84933	84987	875-877	1.000000	79.9
rps19-rpl2_p4	rps19-rpl2	4	TGCTGCTGCAGAATAAACCA	GCGAACCAGAAAAGTTTGGG	20	20	45.0	50.0	58.1	57.9	32.3	0.0	0.225857	84933	84987	811-813	1.000000	79.9
rps19-rpl2_p5	rps19-rpl2	5	TGCTGCTGCAGAATAAACCA	GGCGAACCAGAAAAGTTTGG	20	20	45.0	50.0	58.1	57.9	32.3	0.0	0.225857	84933	84987	812-814	1.000000	79.9
ndhF_p1	ndhF	1	CGATGCAACAACAAGACGTT	ACGATGCACAATACCCCTTC	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.421685	110212	112497	2930-2930	0.125000	41.6
ndhF_p2	ndhF	2	TCGATGCAACAACAAGACGT	ACGATGCACAATACCCCTTC	20	20	45.0	50.0	58.4	58.0	0.0	0.0	0.460918	110212	112497	2931-2931	0.125000	41.3
ndhF_p3	ndhF	3	TTCGATGCAACAACAAGACG	ACGATGCACAATACCCCTTC	20	20	45.0	50.0	57.3	58.0	0.0	0.0	0.699891	110212	112497	2932-2932	0.125000	39.4
ndhF_p4	ndhF	4	CGATGCAACAACAAGACGTT	AACGATGCACAATACCCCTT	20	20	45.0	45.0	57.6	57.5	0.0	0.0	0.890705	110212	112497	2931-2931	0.125000	37.9
ndhF_p5	ndhF	5	TCGATGCAACAACAAGACGT	AACGATGCACAATACCCCTT	20	20	45.0	45.0	58.4	57.5	0.0	0.0	0.929938	110212	112497	2932-2932	0.125000	37.6
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GAAGGGGTATTGTGCATCGT	TTTAGGAGATGACATGCCGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.084828	113019	113887	1010-1092	1.000000	86.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GAAGGGGTATTGTGCATCGT	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.127329	113019	113887	1426-1508	1.000000	86.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GAAGGGGTATTGTGCATCGT	CGCTTGGGCCAATTATTGTG	20	20	50.0	50.0	58.0	58.1	0.0	21.2	0.113158	113019	113887	1398-1480	0.875000	80.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GAAGGGGTATTGTGCATCGT	AATAGCGCTTGGGCCAATTA	20	20	50.0	45.0	58.0	57.9	0.0	21.2	0.104285	113019	113887	1472-1485	0.750000	71.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GAAGGGGTATTGTGCATCGT	TAGCGCTTGGGCCAATTATT	20	20	50.0	45.0	58.0	57.9	0.0	21.2	0.104285	113019	113887	1470-1483	0.750000	71.4
ndhD_p1	ndhD	1	CAATTCAGCAATTGTGGCGT	GATACCCTGGGACGGATGTA	20	20	45.0	55.0	57.9	58.0	7.4	22.2	0.074207	115264	116766	2093-2165	1.000000	84.9
ndhD_p2	ndhD	2	TTGGAGTTGGGACCCAAAAG	GATACCCTGGGACGGATGTA	20	20	50.0	55.0	57.9	58.0	27.9	22.2	0.086024	115264	116766	2189-2261	1.000000	84.7
ndhD_p3	ndhD	3	CAATTCAGCAATTGTGGCGT	GACCGAAGACTGTGTTGGTT	20	20	45.0	50.0	57.9	58.1	7.4	11.2	0.119372	115264	116766	2045-2117	1.000000	84.7
ndhD_p4	ndhD	4	TTGGAGTTGGGACCCAAAAG	GACCGAAGACTGTGTTGGTT	20	20	50.0	50.0	57.9	58.1	27.9	11.2	0.131189	115264	116766	2141-2213	1.000000	84.4
ndhD_p5	ndhD	5	CAATTCAGCAATTGTGGCGT	TGTGAATCTGCCTGTCCAAC	20	20	45.0	50.0	57.9	58.1	7.4	0.0	0.172617	115264	116766	2017-2089	1.000000	84.3
ndhD-psaC_p1	ndhD-psaC	1	ACCTGCTGATATTGGCAAAAC	GCCTGTCCAACCGATTTCTT	21	20	42.9	50.0	57.1	58.5	3.0	0.0	2.339663	116767	116885	251-251	1.000000	66.4
ndhD-psaC_p2	ndhD-psaC	2	GAACCTGCTGATATTGGCAA	GCCTGTCCAACCGATTTCTT	20	20	45.0	50.0	56.1	58.5	0.7	0.0	2.377496	116767	116885	253-253	1.000000	66.0
ndhD-psaC_p3	ndhD-psaC	3	GAACCTGCTGATATTGGCAAA	GCCTGTCCAACCGATTTCTT	21	20	42.9	50.0	56.9	58.5	3.0	0.0	2.614550	116767	116885	253-253	1.000000	64.1
ndhD-psaC_p4	ndhD-psaC	4	CCTGCTGATATTGGCAAAACT	GCCTGTCCAACCGATTTCTT	21	20	42.9	50.0	56.8	58.5	3.0	0.0	2.622778	116767	116885	250-250	1.000000	64.1
ndhD-psaC_p5	ndhD-psaC	5	AACCTGCTGATATTGGCAAAAC	GCCTGTCCAACCGATTTCTT	22	20	40.9	50.0	57.8	58.5	3.0	0.0	2.657700	116767	116885	252-252	1.000000	63.8
rps15-ycf1_p1	rps15-ycf1	1	TCCCAACGCGTTACATAAGG	AGACCCCATTCATTTGCCTG	20	20	50.0	50.0	58.0	58.1	26.1	0.0	0.156308	123042	123407	1475-1497	1.000000	82.1
rps15-ycf1_p2	rps15-ycf1	2	AACACCATGCATTGATGGGT	AGACCCCATTCATTTGCCTG	20	20	45.0	50.0	58.1	58.1	8.9	0.0	0.201994	123042	123407	1338-1360	1.000000	82.0
rps15-ycf1_p3	rps15-ycf1	3	TCCCAACGCGTTACATAAGG	ATTTGCCTGGAAACGACGAT	20	20	50.0	45.0	58.0	58.2	26.1	0.0	0.191720	123042	123407	1464-1486	1.000000	81.9
rps15-ycf1_p4	rps15-ycf1	4	CCGAGCCATAACAAATGGGA	AGACCCCATTCATTTGCCTG	20	20	50.0	50.0	57.9	58.1	6.2	0.0	0.194705	123042	123407	1619-1641	1.000000	81.8
rps15-ycf1_p5	rps15-ycf1	5	TCCCAACGCGTTACATAAGG	CTGGAAACGACGATGGACAA	20	20	50.0	50.0	58.0	58.2	26.1	0.0	0.222182	123042	123407	1458-1477	0.500000	60.6
ycf1_p1	ycf1	1	CCGTTCCATTGACTCGGATT	GGAAGAAGGGGTGGAAAGTG	20	20	50.0	55.0	58.0	58.1	4.3	0.0	0.113118	127978	128097	155-179	0.875000	85.9
ycf1_p2	ycf1	2	CCGTTCCATTGACTCGGATT	AGGGCCCTCTTATCTCTTCC	20	20	50.0	55.0	58.0	57.9	4.3	22.3	0.134929	127978	128097	653-824	1.000000	85.0
ycf1_p3	ycf1	3	CCGTTCCATTGACTCGGATT	GGGCCCTCTTATCTCTTCCT	20	20	50.0	55.0	58.0	57.9	4.3	17.0	0.134929	127978	128097	652-823	1.000000	85.0
ycf1_p4	ycf1	4	CCGTTCCATTGACTCGGATT	TCCGAGCTCAGGTTATGGAA	20	20	50.0	50.0	58.0	58.1	4.3	15.4	0.163535	127978	128097	630-801	1.000000	84.9
ycf1_p5	ycf1	5	CCGTTCCATTGACTCGGATT	GCAGAATACCGTCACCCATT	20	20	50.0	50.0	58.0	58.0	4.3	0.0	0.062562	127978	128097	207-231	0.875000	84.5