Hotspot

rps2

LSC · 14331-15041 · 711 bp

Back to Orchis

Pi 0.00141
MarkerSeek score 59.60
Primer available yes
Label rps2

Feature Metadata

label_name
rps2
feature_type
gene
region
LSC
length_bp
711
variable_sites
1
pi
0.00141
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2_p1 TCAAAGAAAGTTCACCAAAAACGA ATCCTGGTAGAAGGTTCCGT 33.3 50.0 58.0 57.8 855 bp 1.0 46.0
rps2_p2 AGAAAGTTCACCAAAAACGAAC ATCCTGGTAGAAGGTTCCGT 36.4 50.0 56.0 57.8 851 bp 1.0 45.9
rps2_p3 TCACCAAAAACGAACTAATTGGA ATCCTGGTAGAAGGTTCCGT 34.8 50.0 56.9 57.8 844 bp 1.0 45.4
rps2_p4 TCAAAGAAAGTTCACCAAAAACGA TCCTGGTAGAAGGTTCCGTC 33.3 55.0 58.0 58.4 854 bp 1.0 44.4
rps2_p5 AGAAAGTTCACCAAAAACGAAC TCCTGGTAGAAGGTTCCGTC 36.4 55.0 56.0 58.4 850 bp 1.0 44.3