Hotspot

matK

LSC · 1572-3122 · 1551 bp

Pi 0.000645

MarkerSeek score 59.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1551
variable_sites: 1
pi: 0.000645
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	GCACACGACTTTCCCTATGT	TACACATACGCCGTTCTGAC	50.0	50.0	57.9	57.7	1764 bp	1.0	77.1
matK_p2	GCACACGACTTTCCCTATGT	ACGCCGTTCTGACCATATTG	50.0	50.0	57.9	58.3	1757 bp	1.0	76.5
matK_p3	GCACACGACTTTCCCTATGT	CATACGCCGTTCTGACCATA	50.0	50.0	57.9	57.5	1760 bp	1.0	75.3
matK_p4	GCACACGACTTTCCCTATGT	GCCGTTCTGACCATATTGCA	50.0	50.0	57.9	58.6	1755 bp	1.0	74.3
matK_p5	GCACACGACTTTCCCTATGT	CATACGCCGTTCTGACCATAT	50.0	47.6	57.9	58.0	1760 bp	1.0	71.1