Hotspot

rpl2-trnH-GUG

IRa · 151524-151566 · 43 bp

Back to Orchis

Pi 0.0233
MarkerSeek score 65.90
Primer available yes
Label rpl2-trnH-GUG

Feature Metadata

label_name
rpl2-trnH-GUG
feature_type
igs
region
IRa
length_bp
43
variable_sites
1
pi
0.0233
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-trnH-GUG_p1 GTTATCCTGCGCTTGGAAGA CGGATGTAGCCAAGTGGATC 50.0 55.0 58.0 58.1 193 bp 1.0 79.2
rpl2-trnH-GUG_p2 GTTATCCTGCGCTTGGAAGA TGGATCAAGGCAGTGGATTG 50.0 50.0 58.0 57.9 179 bp 1.0 79.2
rpl2-trnH-GUG_p3 GTTATCCTGCGCTTGGAAGA GGATCAAGGCAGTGGATTGT 50.0 50.0 58.0 57.9 178 bp 1.0 79.2
rpl2-trnH-GUG_p4 GTTATCCTGCGCTTGGAAGA GTGGATCAAGGCAGTGGATT 50.0 50.0 58.0 57.9 180 bp 1.0 79.2
rpl2-trnH-GUG_p5 CACAACCCCTTGGGGTTATC CGGATGTAGCCAAGTGGATC 55.0 55.0 58.2 58.1 207 bp 1.0 78.1