Hotspot

trnH-GUG-rpl2

IRb · 83407-83449 · 43 bp

Back to Orchis

Pi 0.0233
MarkerSeek score 65.90
Primer available yes
Label trnH-GUG-rpl2

Feature Metadata

label_name
trnH-GUG-rpl2
feature_type
igs
region
IRb
length_bp
43
variable_sites
1
pi
0.0233
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-rpl2_p1 CGGATGTAGCCAAGTGGATC GTTATCCTGCGCTTGGAAGA 55.0 50.0 58.1 58.0 193 bp 1.0 79.2
trnH-GUG-rpl2_p2 TGGATCAAGGCAGTGGATTG GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 179 bp 1.0 79.2
trnH-GUG-rpl2_p3 GGATCAAGGCAGTGGATTGT GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 178 bp 1.0 79.2
trnH-GUG-rpl2_p4 GTGGATCAAGGCAGTGGATT GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 180 bp 1.0 79.2
trnH-GUG-rpl2_p5 CGGATGTAGCCAAGTGGATC CACAACCCCTTGGGGTTATC 55.0 55.0 58.1 58.2 207 bp 1.0 78.1