Hotspot

rpl20-rps12

LSC · 66794-67576 · 783 bp

Back to Orchis

Pi 0
MarkerSeek score 58.90
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
783
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 GTCAGTCTTGAATGAGCCCC GAAATCTCCCGCTCTTCGAG 55.0 55.0 58.3 58.2 935-936 bp 1.0 76.8
rpl20-rps12_p2 TCAGTCTTGAATGAGCCCCT GAAATCTCCCGCTCTTCGAG 50.0 55.0 58.3 58.2 934-935 bp 1.0 76.1
rpl20-rps12_p3 GTCAGTCTTGAATGAGCCCC TAGAAACGCAAGACAGCCAA 55.0 45.0 58.3 57.8 968-969 bp 1.0 76.0
rpl20-rps12_p4 GTCAGTCTTGAATGAGCCCC TTAGAAACGCAAGACAGCCA 55.0 45.0 58.3 57.8 969-970 bp 1.0 76.0
rpl20-rps12_p5 TCAGTCTTGAATGAGCCCCT TAGAAACGCAAGACAGCCAA 50.0 45.0 58.3 57.8 967-968 bp 1.0 75.4