Hotspot

psbM-trnD-GUC

LSC · 28541-29355 · 815 bp

Back to Orchis

Pi 0.00123
MarkerSeek score 53.70
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
815
variable_sites
1
pi
0.00123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 ATGAACAGCGCAGTAGCAAT AGTTCAATCGGTCAGAGCAC 45.0 50.0 58.3 57.9 925 bp 1.0 77.3
psbM-trnD-GUC_p2 TTTTGGCTGGCTGTTTTGAC AGTTCAATCGGTCAGAGCAC 45.0 50.0 57.7 57.9 979 bp 1.0 76.9
psbM-trnD-GUC_p3 TGAACAGCGCAGTAGCAATA AGTTCAATCGGTCAGAGCAC 45.0 50.0 57.6 57.9 924 bp 1.0 76.4
psbM-trnD-GUC_p4 AAATGAACAGCGCAGTAGCA AGTTCAATCGGTCAGAGCAC 45.0 50.0 58.5 57.9 927 bp 1.0 75.6
psbM-trnD-GUC_p5 AATGAACAGCGCAGTAGCAA AGTTCAATCGGTCAGAGCAC 45.0 50.0 58.5 57.9 926 bp 1.0 75.6