Hotspot

trnG-GCC

LSC · 37850-37920 · 71 bp

Pi 0.0282

MarkerSeek score 67.70

Primer available yes

Label trnG-GCC

Feature Metadata

label_name: trnG-GCC
feature_type: tRNA
region: LSC
length_bp: 71
variable_sites: 2
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC_p1	AGGAGAGTCTTTGGTCTGGG	ACTACACGATCACCGACAAC	55.0	50.0	58.3	57.7	256-274 bp	1.0	75.1
trnG-GCC_p2	GGAGAGTCTTTGGTCTGGGA	ACTACACGATCACCGACAAC	55.0	50.0	58.4	57.7	255-273 bp	1.0	75.0
trnG-GCC_p3	AGGAGAGTCTTTGGTCTGGG	GGGAGGCTATAGGAAAGGGA	55.0	55.0	58.3	57.6	295-312 bp	1.0	74.7
trnG-GCC_p4	AGAGTCTTTGGTCTGGGACT	ACTACACGATCACCGACAAC	50.0	50.0	57.6	57.7	253-271 bp	1.0	74.6
trnG-GCC_p5	GGAGAGTCTTTGGTCTGGGA	GGGAGGCTATAGGAAAGGGA	55.0	55.0	58.4	57.6	294-311 bp	1.0	74.6