Hotspot

clpP1

LSC · 72161-74232 · 2072 bp

Back to Orbea

Pi 0.0454
MarkerSeek score 73.60
Primer available yes
Label clpP1

Feature Metadata

label_name
clpP1
feature_type
gene
region
LSC
length_bp
2072
variable_sites
91
pi
0.0454
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP1_p1 ATTTGTGTGAAATCCGTCTT GTTTCCACATCAAAGTAAAACT 35.0 31.8 53.4 53.2 2140 bp 0.5 20.0
clpP1_p2 ATTTGTGTGAAATCCGTCTT TCCACATCAAAGTAAAACTTAGTTCT 35.0 30.8 53.4 57.0 2137 bp 0.5 20.0
clpP1_p3 ATTTGTGTGAAATCCGTCTT GTTTCCACATCAAAGTAAAACTT 35.0 30.4 53.4 54.0 2140 bp 0.5 20.0
clpP1_p4 ATTTGTGTGAAATCCGTCTT TCCACATCAAAGTAAAACTTAGT 35.0 30.4 53.4 53.9 2137 bp 0.5 20.0
clpP1_p5 ATTTGTGTGAAATCCGTCTT TCCACATCAAAGTAAAACTTAGTTC 35.0 32.0 53.4 55.8 2137 bp 0.5 20.0