Hotspot

trnN-GUU-ycf1

IRb · 110706-111737 · 1032 bp

Back to Orbea

Pi 0.027
MarkerSeek score 49.30
Primer available yes
Label trnN-GUU-ycf1

Feature Metadata

label_name
trnN-GUU-ycf1
feature_type
igs
region
IRb
length_bp
1032
variable_sites
8
pi
0.027
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.29
markerseek_score
49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ycf1_p1 CAATTGTGACCTCTCGGGAG AATGGGTTCCGGTATTCTGC 55.0 50.0 58.0 57.9 1230-1939 bp 1.0 84.7
trnN-GUU-ycf1_p2 AGAATGATGGGGATAGCGGA AATGGGTTCCGGTATTCTGC 50.0 50.0 58.0 57.9 1502-2211 bp 1.0 84.5
trnN-GUU-ycf1_p3 AGATAAACGCGGCTTCACAT AATGGGTTCCGGTATTCTGC 45.0 50.0 58.0 57.9 1728-2437 bp 1.0 84.4
trnN-GUU-ycf1_p4 ATAAACGCGGCTTCACATCT AATGGGTTCCGGTATTCTGC 45.0 50.0 58.0 57.9 1726-2435 bp 1.0 84.4
trnN-GUU-ycf1_p5 TTGTGACCTCTCGGGAGAAT AATGGGTTCCGGTATTCTGC 50.0 50.0 58.1 57.9 1227-1936 bp 1.0 84.4