Hotspot

trnR-ACG-trnN-GUU

IRb · 110208-110633 · 426 bp

Back to Orbea

Pi 0.0117
MarkerSeek score 61.90
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
426
variable_sites
5
pi
0.0117
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG GCTCATATTGGTCGTGGGTT 55.0 50.0 57.8 58.0 595-622 bp 1.0 78.6
trnR-ACG-trnN-GUU_p2 CAGAGGATTAGAGCACGTGG ATTGGTCGTGGGTTGAGAAC 55.0 50.0 57.8 58.1 589-616 bp 1.0 78.1
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 518 bp 1.0 77.8
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG CGATTGGTCGTAGGTTCGAA 55.0 50.0 57.8 57.7 522 bp 1.0 76.9
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG TTCCTCAGTAGCTCAGTGGT 55.0 50.0 57.8 57.7 561 bp 1.0 76.5