Hotspot

rps19-rpl2

IRb · 86499-86522 · 24 bp

Back to Orbea

Pi 0.0417
MarkerSeek score 71.70
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
IRb
length_bp
24
variable_sites
1
pi
0.0417
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 GATGCTCGGGACCAAGTTAC TTGATTCTTCGTCGCCGTAG 55.0 50.0 58.3 58.1 164-211 bp 1.0 76.9
rps19-rpl2_p2 GATGCTCGGGACCAAGTTAC CACAACCCCTTGGGGTTATC 55.0 55.0 58.3 58.2 231-278 bp 1.0 76.3
rps19-rpl2_p3 AGATGCTCGGGACCAAGTTA TTGATTCTTCGTCGCCGTAG 50.0 50.0 58.4 58.1 165-212 bp 1.0 76.2
rps19-rpl2_p4 TAGATGCTCGGGACCAAGTT TTGATTCTTCGTCGCCGTAG 50.0 50.0 58.4 58.1 166-213 bp 1.0 76.2
rps19-rpl2_p5 GATGCTCGGGACCAAGTTAC TTGGGGTTATCCTGCACTTG 55.0 50.0 58.3 57.8 222-269 bp 1.0 75.8