Hotspot

rps11-rpl36

LSC · 81701-81811 · 111 bp

Back to Orbea

Pi 0.027
MarkerSeek score 67.20
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
111
variable_sites
3
pi
0.027
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 CGTGACCCAATACGTCCATT GCGGGGTCGAATTAGAGTAA 50.0 50.0 58.0 57.1 225 bp 1.0 73.9
rps11-rpl36_p2 GCACTCTTACGTGACCCAAT GCGGGGTCGAATTAGAGTAA 50.0 50.0 57.9 57.1 234 bp 1.0 73.3
rps11-rpl36_p3 CGTGACCCAATACGTCCATT GGCGGGGTCGAATTAGAGTA 50.0 55.0 58.0 59.0 226 bp 1.0 73.2
rps11-rpl36_p4 CCTTTGGGATTCTACGTGCA GCGGGGTCGAATTAGAGTAA 50.0 50.0 57.9 57.1 251 bp 1.0 73.1
rps11-rpl36_p5 GCACTCTTACGTGACCCAAT GGCGGGGTCGAATTAGAGTA 50.0 55.0 57.9 59.0 235 bp 1.0 72.5