Hotspot

trnH-GUG-psbA

LSC · 106-447 · 342 bp

Back to Orbea

Pi 0.0449
MarkerSeek score 75.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
342
variable_sites
14
pi
0.0449
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 410-411 bp 1.0 85.1
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 424-425 bp 1.0 85.0
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 423-424 bp 1.0 85.0
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 425-426 bp 1.0 85.0
trnH-GUG-psbA_p5 AAAACAAAAGGGCGAACGAC TTCCCTCTAGACCTAGCTGC 45.0 55.0 57.8 57.9 474 bp 0.5 57.8