Hotspot

ycf1

Genome · 133089-135941 · 2853 bp

Back to Opuntia

Pi 0.0097

MarkerSeek score 53.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 2853
variable_sites: 62
pi: 0.0097
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGCGTGCGCATCTATTTATC	GGAACGATGATGCATCTTATAGT	50.0	39.1	57.9	56.3	2164-3000 bp	0.333333	19.4
ycf1_p2	CGCGTGCGCATCTATTTATC	GGAACGATGATGCATCTTAT	50.0	40.0	57.9	53.2	2164-3000 bp	0.333333	19.0
ycf1_p3	GCGTGCGCATCTATTTATCT	TGGAACGATGATGCATCTTA	45.0	40.0	56.5	54.6	2164-3000 bp	0.333333	18.3
ycf1_p4	GCGTGCGCATCTATTTATCT	TGGAACGATGATGCATCTTAT	45.0	38.1	56.5	55.2	2164-3000 bp	0.333333	18.0
ycf1_p5	CGCGTGCGCATCTATTTATC	GGAACGATGATGCATCTTATAG	50.0	40.9	57.9	54.6	2164-3000 bp	0.333333	18.0