Hotspot

petD

Genome · 20591-21860 · 1270 bp

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Pi 0.00157

MarkerSeek score 60.40

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: Genome
length_bp: 1270
variable_sites: 6
pi: 0.00157
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACTTTTGAAGCGACTATTCCCT	TGGACATTTTCCTTCAACTCCA	40.9	40.9	57.7	57.8	1422-1428 bp	1.0	45.0
petD_p2	CACTTTTGAAGCGACTATTCCC	TGGACATTTTCCTTCAACTCCA	45.5	40.9	57.7	57.8	1423-1429 bp	1.0	44.5
petD_p3	ACTTTTGAAGCGACTATTCCCT	GGACATTTTCCTTCAACTCCAC	40.9	45.5	57.7	57.5	1421-1427 bp	1.0	42.3
petD_p4	CACTTTTGAAGCGACTATTCCC	GGACATTTTCCTTCAACTCCAC	45.5	45.5	57.7	57.5	1422-1428 bp	1.0	41.9
petD_p5	TCCGAATAGGCCCATAATTTCA	TGGACATTTTCCTTCAACTCCA	40.9	40.9	57.3	57.8	1476-1482 bp	1.0	41.5